hyperion imaging system Search Results


imaging mass cytometry  (fluidigm)
98
fluidigm imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by fluidigm, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging system hyperion 3000  (Bruker Corporation)
90
Bruker Corporation imaging system hyperion 3000
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging System Hyperion 3000, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fpa-ftir spectral images using bruker hyperion 2000 fpa-ftir system  (Bruker Corporation)
90
Bruker Corporation fpa-ftir spectral images using bruker hyperion 2000 fpa-ftir system
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Fpa Ftir Spectral Images Using Bruker Hyperion 2000 Fpa Ftir System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging spectrometer hyperion  (Bruker Corporation)
90
Bruker Corporation imaging spectrometer hyperion
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Spectrometer Hyperion, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imaging spectrometer hyperion/product/Bruker Corporation
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hyperion images  (geological survey network)
90
geological survey network hyperion images
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Images, supplied by geological survey network, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion ir imaging unit  (Bruker Corporation)
90
Bruker Corporation hyperion ir imaging unit
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Ir Imaging Unit, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eo-1 satellite image  (geological survey network)
90
geological survey network eo-1 satellite image
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Eo 1 Satellite Image, supplied by geological survey network, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fpa-based μftir imaging microscope bruker hyperion 3000  (Bruker Corporation)
90
Bruker Corporation fpa-based μftir imaging microscope bruker hyperion 3000
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Fpa Based μftir Imaging Microscope Bruker Hyperion 3000, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fpa-based μftir imaging microscope bruker hyperion 3000/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
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hyperion 300 imager  (Bruker Corporation)
90
Bruker Corporation hyperion 300 imager
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion 300 Imager, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hyperion 300 imager/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
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hyperion ir imaging system  (Bruker Corporation)
90
Bruker Corporation hyperion ir imaging system
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Ir Imaging System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hyperion ir imaging system/product/Bruker Corporation
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hyperion view images  (VIDA Diagnostics)
90
VIDA Diagnostics hyperion view images
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion View Images, supplied by VIDA Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single-reflection diamond atr accessory of a bruker vertex 70 spectrometer/hyperion 300 imager  (Bruker Corporation)
90
Bruker Corporation single-reflection diamond atr accessory of a bruker vertex 70 spectrometer/hyperion 300 imager
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Single Reflection Diamond Atr Accessory Of A Bruker Vertex 70 Spectrometer/Hyperion 300 Imager, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Journal: Science translational medicine

Article Title: Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation.

doi: 10.1126/scitranslmed.abn2263

Figure Lengend Snippet: Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Article Snippet: Imaging mass cytometry of tissue sections Formalin-fixed, paraffin-embedded skin biopsy tissue sections from lesional skin of patients with SCLE or DLE were analyzed using a Hyperion imaging cytometry by time of flight system (CyTOF) (Fluidigm) as previously described (34) with modifications of the antibody panel.

Techniques: Control, Staining, Expressing, Generated, Imaging, Mass Cytometry